WP4:Tight-junction monoclonal antibodies

The overall aim of this workpackage is to establish a back-up anti-receptor antibody pipeline. The specific objectives are:

  • To generate and isolate murine monoclonal antibodies targeting tight junction proteins involved in HCV entry

  • Screen these novel antibodies for antiviral activity, including an assessment of breadth and potency

  • Humanise at least one monoclonal antibody that demonstrates the highest potency and breadth

  • Determine the in vitro and in vivo neutralizing activity of the humanised antibody